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Programme (0)

FRIDAY 19/1/2018

 

10:00-10:45

Welcome to the course (P. Lenzi)

10:45-11:00

Coffee break

11:00-13:00

Lesson: Introduction to transmission electron microscopy  (TEM) (A. Falleni)

 

Lesson: Introduction to scanning electron microscopy (SEM) (F. Verni)

15:00-16:00

Lesson: Sample processing for TEM analysis (P. Lenzi)

16:15-17:30

Lesson: Sample fixation for SEM analysis (F. Verni)

 

 

SATURDAY 20/1/2018 

 

9:30-11:00

Lesson: Sample processing for TEM: Advanced specimens preparation (P. Lenzi)

11:00-11:30

Coffee break

11:00- 13:00

Lessons: TEM applications in amphetamines effects (G. Lazzeri)

 

 

MONDAY 22/1/2018

 

9:30-10:30

Lesson/Practice: Trimming of the blocks and cut of ultrathin sections with the ultramicrotome  (A. Falleni )

10:30-11:00

Practice: Fixation of samples for TEM  I (A Salvetti, P. Lucchesi )

11:00-11:30

Coffee break

11:30-12:30

Practice: Sample processing for SEM I (Sample preparation  for fixing (cell and tissue) (F. Verni, L. Modeo )

12:30-13:00

Practice: Fixation of samples for TEM II  (A. Salvetti, P. Lucchesi)

14:00-17:30 

Practice: Sample processing for TEM I (dehydratation and infiltration with epoxy resin) (A. Salvetti, L. Rossi)

 

 

THUESDAY 23/1/2018

 

9:00-9:30

Practice: Sample processing for TEM II (infiltration with 100% epoxy resin) (A. Salvetti, L. Rossi)

9:30-10:30

Practice: Sample processing for SEM II (Fixation and dehydratation) (F. Verni, L. Modeo)

10:30-11:00

Coffee break

11:00-13:00

Practice: Sample processing for SEM III (Critical Point) (F. Verni, L. Modeo)

15:00-16:00

Practice: Sample preparation for TEM III (final embedding in epoxy resin) (A. Salvetti, L. Rossi)

16:00-17:30

Practice: Sample processing for SEM IV (Covering) (F. Verni, L. Modeo)

 

 

WEDNESDAY 24/1/2018

 

9:30-11:00

Lesson:  Immunolobeling techniques in transmission electron microscopy (P. Lenzi)

11:00-11:30

Coffee break

11:30-13:00

Practice: Sample observation at scanning electron microscope (F. Verni, L. Modeo)

15:00-17:30

Lesson: The contribution of TEM to solving isssues in the reproductive biology of lower invertebrates (A. Falleni)

 

Lesson: 

Morphological and ultrastructural studies in different experimental conditions: personal research experience. (R. Ruffoli)

 

 

THURSDAY  25/1/2018

 

9:00-11:00

Lesson/Practice: The electron beam and image formation in electron microscopy.( P. Lucchesi)

11:00-11:30

Coffee break

11:30-13:00

Practice: Cut of ultrathin sections with the ultramicrotome equipped with a diamond knife and section collection on copper grids. Staining of ultrathin sections  (A. Falleni, P. Lucchesi)

15:00-17:00

Practice: Sample observation at trasmission electron microscope (P. Lenzi, A. Falleni, A. Salvetti)

17:00-17:30

Practice: Immunolobeling experiment I (L. Riskalyn)

 

 

FRIDAY 26/1/2018

 

9:30-10:30

Practice: Immunolobeling experiment II (L. Riskalyn)

10:30-11:00

Coffee break

11:00-13:00

Lesson: The use of SEM in paleopathology: the museal experience . (V. Giuffra, G. Natale)

12:00-13:00

 

 

Free afternoon

18:30

Guided tour of the Anatomy museum " Filippo Civinini"

 

 

SATURDAY 27/1/2018

 

9:30-10:30

Final evaluation test

10:30-11:00

Record of credits